イワミ　ノリオ   岩見　徳雄    所属   理工学部 総合理工学科    職種   准教授
言語種別	英語
発行・発表の年月	2019/03
形態種別	学術雑誌
査読	査読あり
標題	Development of One cell or One individual Direct PCR of Protozoan or Metazoan 18S rRNA Gene for Molecular Ecology
執筆形態	共著
掲載誌名	Indian Journal of Ecology
掲載区分	国外
出版社・発行元	Indian Ecological Society
巻・号・頁	46(3),pp.486-492
総ページ数	7
著者・共著者	Watcharapong Thakong, Duangduean Yuenyongkirimard, Kazuya Shimizu, Norio Iwami, Niwooti Whangchai, Rameshprabu Ramaraj and Tomoaki Itayama
概要	The new method for one cell or one individual direct PCR to build a local DNA database of protozoans and metazoans for the molecular ecological studies was developed. We applied a nested PCR for 18S rRNA gene of the isolated rotifer individual and a protozoan cell. We determined the sequence of each PCR amp icon for the rotifer individual or the protozoa. As a result, using the developed new method, we could correctly determine the partial sequence of 18r RNA genes of 17 samples of ciliates and rotifers in 20 samples from natural ponds and activated sludge systems.