タシロ ミツル
田代 充 所属 理工学部 総合理工学科 職種 教授 |
|
言語種別 | 英語 |
発行・発表の年月 | 2022/03 |
形態種別 | 学術雑誌 |
査読 | 査読あり |
標題 | Development of 1H{19F} Saturation Transfer Difference Experiments for Detection of a Fluorinated Compound Bound to Proteins |
執筆形態 | 共著 |
掲載誌名 | Anal. Sci. |
掲載区分 | 国内 |
出版社・発行元 | 日本分析化学会 |
総ページ数 | 3 |
担当区分 | 最終著者,責任著者 |
著者・共著者 | FURIHATA and Mitsuru TASHIRO, |
概要 | The 1H{19F} saturation transfer difference (STD) experiment presented here incorporates the WATERGATE W5 sequence to observe protein-ligand interactions in a human serum albumin (HSA)-fleroxacin complex. In conventional STD experiments, 1H of proteins are first saturated, and the ligands bound to these proteins are then observed. The method proposed here reverses this process: fluorine atoms in fleroxacin are selectively saturated, and saturation transfer then occurs to protons of fleroxacin as well as to those of HSA. The combined use of the present 1H{19F} STD and conventional STD methods is expected to provide better insight in the analysis of the role of fluorine atoms in a fluorinated compound. |