タシロ ミツル
  田代 充
   所属   理工学部 総合理工学科(生命科学・化学系)
   職種   教授
言語種別 英語
発行・発表の年月 2020/07
形態種別 学術雑誌
査読 査読あり
標題 Identification of Binding Epitopes of a Fluorinated Compound Bound to Proteins Using 1H and 19F NMR Spectroscopy
執筆形態 共著
掲載区分国内
出版社・発行元 日本分析化学会
巻・号・頁 36(7),pp.881-883
総ページ数 3
担当範囲 NMR測定、執筆
著者・共著者 Kazuo FURIHATA and Mitsuru TASHIRO
概要 1H/19F NMR-based screening methods were applied to a human serum albumin-fleroxacin complex. Fleroxacin contains three fluorine atoms in a molecule, which is suitable as a model fluorinated compound for NMR analysis with 1H and 19F detection. The 19F{1H} and 1H{1H} saturation transfer difference spectra were acquired and the 1H/19F spin-lattice relaxation rates were measured with and without any selective irradiation of protein resonance to identify the binding epitopes of fleroxacin. Because several 1H signals of fleroxacin resonated close to water, its precise signal intensities were unavailable. The 19F NMR-based screening methods successfully provide complementary information, indicating its importance in the analysis of fluorinated compounds.