タシロ　ミツル   田代　充    所属   理工学部 総合理工学科    職種   教授
言語種別	英語
発行・発表の年月	2023
形態種別	学術雑誌
査読	査読あり
標題	Application of 31P NMR spectroscopy to analyze the affinity of 3’-AMP bound to proteins
執筆形態	共著
掲載誌名	Current Topics in Analytical Chemistry
掲載区分	国外
出版社・発行元	Research Trends
巻・号・頁	14,pp.67-71
総ページ数	5
担当範囲	NMR測定、執筆
担当区分	責任著者
著者・共著者	K. Furihata, M. Tashiro
概要	Various 31P NMR techniques, including 31P-T1, 31P-T2, 31P-diffusion ordered spectroscopy and 31P{1H}saturation transfer difference techniques, were applied to analyze the affinity of adenosine 3’-monophosphate (3’-AMP) to human serum albumin. To date, no structures of the complex comprising 3’-AMP and human serum albumin have been deposited in Protein Data Bank. Because the 31P nucleus is a selective marker of phosphorylated compounds, its observation by NMR spectroscopy can be an effective method to investigate these molecular interactions in the solution state. In the present system, the 1H/31P-T2 measurements provided the sensitive results that confirmed complex formation, and simple comparisons of the 31P signal decay using the pulse sequence to measure 31P-T2 reflected these molecular interactions. The 31P{1H} saturation transfer difference experiment was also applied to confirm 3’-AMP bound to proteins.