タシロ　ミツル   田代　充    所属   理工学部 総合理工学科    職種   教授
言語種別	英語
発行・発表の年月	2022/03
形態種別	学術雑誌
査読	査読あり
標題	Development of 1H{19F} Saturation Transfer Difference Experiments for Detection of a Fluorinated Compound Bound to Proteins
執筆形態	共著
掲載誌名	Anal. Sci.
掲載区分	国内
出版社・発行元	日本分析化学会
総ページ数	3
担当区分	最終著者,責任著者
著者・共著者	FURIHATA and Mitsuru TASHIRO,
概要	The 1H{19F} saturation transfer difference (STD) experiment presented here incorporates the WATERGATE W5 sequence to observe protein-ligand interactions in a human serum albumin (HSA)-fleroxacin complex. In conventional STD experiments, 1H of proteins are first saturated, and the ligands bound to these proteins are then observed. The method proposed here reverses this process: fluorine atoms in fleroxacin are selectively saturated, and saturation transfer then occurs to protons of fleroxacin as well as to those of HSA. The combined use of the present 1H{19F} STD and conventional STD methods is expected to provide better insight in the analysis of the role of fluorine atoms in a fluorinated compound.